کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1939061 | 1050753 | 2006 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MafA differentiates rat intestinal cells into insulin-producing cells MafA differentiates rat intestinal cells into insulin-producing cells](/preview/png/1939061.png)
Recent studies have suggested that basic leucine zipper transcription factor MafA has a crucial role in pancreatic β-cell-specific insulin gene transcription. Thus, we investigated whether MafA overexpression in the intestine induces insulin production in small-intestinal epithelial cells in vivo. Recombinant adenovirus containing MafA gene (Ad-MafA) was prepared and administered orally to streptozocin-treated diabetic rats. Insulin gene expression was observed in the intestine by RT-PCR analysis, and then insulin protein was detected by immunohistochemical analysis after Ad-MafA administration. Furthermore, MafA overexpression in the intestine increased plasma insulin levels and ameliorated hyperglycemia. These results indicate that MafA overexpression in the intestine induces intestinal epithelial cells newly to produce and release insulin.
Journal: Biochemical and Biophysical Research Communications - Volume 349, Issue 1, 13 October 2006, Pages 136–143