کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939187 1050756 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aptamer selection based on inhibitory activity using an evolution-mimicking algorithm
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Aptamer selection based on inhibitory activity using an evolution-mimicking algorithm
چکیده انگلیسی

In order to efficiently select aptamers that bind to and inhibit proteins, we developed a method that involves screening DNA aptamers based on their inhibitory activities using an evolution-mimicking algorithm after the pre-selection by SELEX. The value of this method was demonstrated by the identification of an inhibitor of Taq DNA polymerase in a unique single-stranded DNA library, which was expected to form a G-quartet structure. This method consists of selection via an inhibition assay, sequence shuffling, and mutation in silico. After six rounds of selection, the inhibitory activities of the aptamers had evolved significantly. This demonstrates the utility of this strategy for screening aptamers based on their inhibitory actions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 347, Issue 1, 18 August 2006, Pages 226–231
نویسندگان
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