کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1939375 | 1050759 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: KSHV RTA induces a transcriptional repressor, HEY1 that represses rta promoter KSHV RTA induces a transcriptional repressor, HEY1 that represses rta promoter](/preview/png/1939375.png)
Kaposi’s sarcoma-associated herpesvirus (KSHV) is a γ-herpesvirus that is frequently associated with the formation of tumors like Kaposi’s sarcoma and primary effusion lymphomas. KSHV RTA is required for lytic replication/reactivation in KSHV-infected cells. We developed an inducible RTA expression cell line to search for RTA-inducible genes. Using DNA chip technology, we showed that hey1, a basic helix-loop-helix-containing transcription factor, increased three- to fourfold in this line. In a luciferase reporter system, RTA activated the hey1 promoter in several cell lines; conversely, HEY1 repressed the rta promoter. Chromatin immunoprecipitation assays indicated that HEY did not bind the rta promoter directly, although it may repress rta expression as part of a complex. Thus, hey1 may help to downregulate RTA after its burst of expression during lytic replication/reactivation. Similar repression mechanisms may downregulate immediate-early gene expression in other herpesviruses, although different transcription factors are probably involved.
Journal: Biochemical and Biophysical Research Communications - Volume 345, Issue 1, 23 June 2006, Pages 410–418