کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939486 1050761 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Coupled expression of MhpE aldolase and MhpF dehydrogenase in Escherichia coli
چکیده انگلیسی

MhpE (4-hydroxy-2-ketovalerate aldolase) and MhpF [acetaldehyde dehydrogenase (acylating)] are responsible for the last two reactions in the 3-(3-hydroxyphenyl)propionate (3-HPP) catabolic pathway in Escherichia coli, which is homologous to the meta-cleavage pathway in Pseudomonas species. Here, we report that the MhpE aldolase is associated with the MhpF dehydrogenase and that MhpF is indispensable for the folding of MhpE. Moreover, our results suggest that the mhpF and mhpE genes are translationally coupled through a reinitiation mechanism. This reinitiation mechanism may function in ensuring that the expression of mhpE occurs only when MhpF is available for the formation of a complex.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 346, Issue 3, 4 August 2006, Pages 1009–1015
نویسندگان
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