کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939519 1050762 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assay and characterization of a strong promoter element from B. subtilis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Assay and characterization of a strong promoter element from B. subtilis
چکیده انگلیسی

A new strong promoter fragment isolated from Bacillus subtilis was identified and characterized. Using the heat stable β-galactosidase as reporter, the promoter fragment exhibited high expression strength both in Escherichia coli and B. subtilis. The typical prokaryotic promoter conservation regions were found in the promoter fragment and the putative promoter was identified as the control element of yxiE gene via sequencing assay and predication of promoter. To further verify and characterize the cloned strong promoter, the putative promoter was sub-cloned and the β-Gal directed by the promoters was high-level expressed both in E. coli and B. subtilis. By means of the isolated promoter, an efficient expression system was developed in B. subtilis and the benefit and usefulness was demonstrated through expression of three heterologous and homogenous proteins. Thus, we identified a newly strong promoter of B. subtilis and provided a robust expression system for genetic engineering of B. subtilis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 1, 2 March 2007, Pages 90–95
نویسندگان
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