کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1939523 | 1050762 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A neutralizable epitope is induced on HGF upon its interaction with its receptor cMet
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
A new conformational neutralizable epitope is created on heptocyte growth factor (HGF), when it interacts with its receptor, cMet. By immunizing rabbits with HGF–cMet complex, we successfully generated a monoclonal antibody (SFN68) that inhibits HGF–cMet interaction, and blocks the biological function mediated by HGF. To define the epitope, we screened out an epitope-mimicking peptide, KSLSRHDHIHHH, from a phage display of combinatorial peptide library. In molecular mimicry this peptide bound to cMet and inhibited HGF–cMet interaction. No humoral response was induced to this epitope-mimicking peptide when immunization was done with HGF alone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 1, 2 March 2007, Pages 115–121
Journal: Biochemical and Biophysical Research Communications - Volume 354, Issue 1, 2 March 2007, Pages 115–121
نویسندگان
Kisu Kim, Youngmi Hur, En-Kyung Ryu, Jung-Hyo Rhim, Cha Yong Choi, Cheol-Min Baek, Jae-Ho Lee, Junho Chung,