کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939668 1050764 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cyclic stretch translocates the α2-subunit of the Na pump to plasma membrane in skeletal muscle cells in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cyclic stretch translocates the α2-subunit of the Na pump to plasma membrane in skeletal muscle cells in vitro
چکیده انگلیسی

The Na+–K+-ATPase and its regulation is important for maintaining membrane potential and transmembrane Na+ gradient in all skeletal muscle cells and thus is essential for cell survival and function. In our previous study, cyclic stretch activated the Na pump in cultured skeletal muscle cells. Presently, we investigated whether this stimulation was the result of translocation of Na+–K+-ATPase from endosomes to the plasma membrane, and also evaluated the role of phosphatidylinositol 3-kinase (PI 3-kinase), the activation of which initiated vesicular trafficking and targeting of proteins to specific cell compartments. Skeletal muscle cells were stretched at 25% elongation continuous for 24 h using the Flexercell Strain Unit. The plasma membrane and endosome fractions were isolated and Western blotted to localize the Na+–K+-ATPase α1- and α2-subunit protein. The results showed stretch increased Na+–K+-ATPase α1- and α2-subunit protein expression in plasma membrane fractions and decreased it in endosomes. The α2-subunit had a more dynamic response to mechanical stretch. PI 3-kinase inhibitors (LY294002) blocked the stretch-induced translocation of the Na+–K+-ATPase α2-subunit, while LY294002 had no effect on the transfer of α1-subunit. We concluded that cyclic stretch mainly stimulated the translocation of the α2-subunit of Na+–K+-ATPase from endosomes to the plasma membrane via a PI 3-kinase-dependent mechanism in cultured skeletal muscle cells in vitro, which in turn increased the activity of the Na pump.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 348, Issue 2, 22 September 2006, Pages 750–757
نویسندگان
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