کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939751 1050766 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Unique CCT repeats mediate transcription of the TWIST1 gene in mesenchymal cell lines
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Unique CCT repeats mediate transcription of the TWIST1 gene in mesenchymal cell lines
چکیده انگلیسی

TWIST1, a basic helix–loop–helix transcription factor, plays critical roles in embryo development, cancer metastasis and mesenchymal progenitor differentiation. Little is known about transcriptional regulation of TWIST1 expression. Here we identified DNA sequences responsible for TWIST1 expression in mesenchymal lineage cell lines. Reporter assays with TWIST1 promoter mutants defined the −102 to −74 sequences that are essential for TWIST1 expression in human and mouse mesenchymal cell lines. Tandem repeats of CCT, but not putative CREB and NF-κB sites in the sequences substantially supported activity of the TWIST1 promoter. Electrophoretic mobility shift assay demonstrated that the DNA sequences with the CCT repeats formed complexes with nuclear factors, containing, at least, Sp1 and Sp3. These results suggest critical implication of the CCT repeats in association with Sp1 and Sp3 factors in sustaining expression of the TWIST1 gene in mesenchymal cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 352, Issue 4, 26 January 2007, Pages 925–931
نویسندگان
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