کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1939821 | 1050768 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular cloning of pigGnT-I and I.2: An application to xenotransplantation Molecular cloning of pigGnT-I and I.2: An application to xenotransplantation](/preview/png/1939821.png)
Xenotransplantation is one of the most attractive solutions for the current worldwide shortage of organs. The knocking out of α1,3-galactosyltransferase in pigs resulted in a drastic reduction in xenoantigenicity. However, more recent studies indicate that other xeno-antigens, so-called non-Gal antigens, will also need to be downregulated. In this study, pig N-acetylglucosaminyltransferase I (GnT-I), a key enzyme that initiates the biosynthesis of hybrid- and complex-type N-linked sugar chains, was isolated and the pigGnT-I.2 specific for the O-linked sugar chain was also isolated. Point mutants, pigGnT-I(123) and pigGnT-I(320), were subsequently constructed. While pigGnT-I(123) shows an indistinct dominant negative effect for endogenous GnT-I in pig cells, pigGnT-I(320) had a drastic effect. In addition, in the case of pig cell transfectants with pigGnT-I(320), cell surface carbohydrate structures were significantly altered and its antigenicity to human serum was reduced. Consequently, pigGnT-I(320) appears to be potentially useful in xenotransplantation by remodeling the carbohydrate structures on pig cells.
Journal: Biochemical and Biophysical Research Communications - Volume 343, Issue 3, 12 May 2006, Pages 677–683