کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939939 1050770 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitochondrial cytochrome c reacts with nitric oxide via S-nitrosation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mitochondrial cytochrome c reacts with nitric oxide via S-nitrosation
چکیده انگلیسی

The present study demonstrates that mitochondrial cytochrome c reacts with the thiol-reacting agent N-ethylmaleimide (NEM) to produce a one NEM-adducted cytochrome c. Mitochondrial cytochrome c also reacts with 5,5′-dithio-bis-(2-nitrobenzoic acid) and 1-chloro-2,4-dinitrobenzene in a manner prevented with NEM or iodoacetic acid (IAA). NEM-treated cytochrome c has lower reducibility and lower function to support mitochondrial oxygen consumption. These findings suggest that mitochondrial cytochrome c contains a reactive thiol that is involved in the functions of cytochrome c for mitochondria. Nitric oxide reacts with the cytochrome c thiol to generate S-nitroso (SNO)-cytochrome c in a manner prevented with NEM or IAA. SNO-cytochrome c has lower reducibility and function to support mitochondrial oxygen consumption, similar to NEM-treated cytochrome c.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 3, 14 April 2006, Pages 991–995
نویسندگان
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