کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1939949 1050771 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pigment epithelium-derived factor protects retinal pigment epithelium from oxidant-mediated barrier dysfunction
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Pigment epithelium-derived factor protects retinal pigment epithelium from oxidant-mediated barrier dysfunction
چکیده انگلیسی

Retinal pigment epithelium (RPE) cells form a monolayer at the blood–retina barrier between the retina and choriocapillaries. The barrier function may be damaged by multiple stresses to the cell, including the repeated exposure to oxidants that are generated by photoreceptor cell turnover. The purpose of our study was to document the protective effect of pigment epithelium-derived factor (PEDF), a tropic factor produced by the RPE, on H2O2-induced RPE barrier dysfunction. When assayed by a FITC-labeled dextran transepithelial flux, the increased permeability of the RPE barrier (induced by H2O2) was prevented by PEDF pretreatment. To further explore the mechanism leading to this permeability change, we investigated the distribution of cytoskeleton and junctional proteins. The redistribution of the two junctional proteins occludin, and N-cadherin and actin reorganization in RPE, induced by H2O2, can be prevented by PEDF pretreatment. PEDF can also prevent H2O2-induced stress kinase p38/27-kDa heat shock protein signaling which is known to mediate actin rearrangement. These findings indicated that PEDF can stabilize actin, maintain normal membrane occludin and N-cadherin structure, and preserve the barrier function of RPE cells against oxidative stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 2, 7 April 2006, Pages 372–378
نویسندگان
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