کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940004 1050772 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
P53 status influences regulation of HSPs and ribosomal proteins by PDTC and radiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
P53 status influences regulation of HSPs and ribosomal proteins by PDTC and radiation
چکیده انگلیسی

Pyrrolidine dithiocarbamate (PDTC) is a thiol-containing compound that can act under varying conditions as an anti-oxidant or pro-oxidant. Utilizing microarrays, we determined the effect of PDTC +/− ionizing radiation (IR) on the expression of heat shock protein (HSP) genes in isolated B6/129 wild-type (WT) and p53−/− spleen cells. Extremely significant microarrays demonstrated that PDTC, but not IR, markedly up-regulated the expression of the majority of detectable HSP genes in WT and many to a significantly greater degree in p53−/− deficient cells. Determination of the glutathione/glutathione disulfide ratio indicated that PDTC was acting as a pro-oxidant under these conditions. From these data we conclude that the clinical use of “antioxidants” with radiotherapy or chemotherapy must be very carefully based on knowledge of the p53 status of their intended normal and tumor target cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 343, Issue 2, 5 May 2006, Pages 435–442
نویسندگان
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