کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940032 1050772 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and characterization of the adipokinetic hormone receptor from the cockroach Periplaneta americana
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cloning and characterization of the adipokinetic hormone receptor from the cockroach Periplaneta americana
چکیده انگلیسی

Cockroaches have long been used as insect models to investigate the actions of biologically active neuropeptides. Here, we describe the cloning and functional expression in Chinese hamster ovary cells of an adipokinetic hormone (AKH) G protein-coupled receptor from the cockroach Periplaneta americana. This receptor is only activated by various insect AKHs (we tested eight) and not by a library of 29 other insect or invertebrate neuropeptides and nine biogenic amines. Periplaneta has two intrinsic AKHs, Pea-AKH-1, and Pea-AKH-2. The Periplaneta AKH receptor is activated by low concentrations of both Pea-AKH-1 (EC50, 5 × 10−9 M), and Pea-AKH-2 (EC50, 2 × 10−9 M). Insects can be subdivided into two evolutionary lineages, holometabola (insects with a complete metamorphosis during development) and hemimetabola (incomplete metamorphosis). This paper describes the first AKH receptor from a hemimetabolous insect.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 343, Issue 2, 5 May 2006, Pages 638–643
نویسندگان
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