کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940065 1050773 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a functional nuclear export signal in the green fluorescent protein asFP499
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of a functional nuclear export signal in the green fluorescent protein asFP499
چکیده انگلیسی

The green fluorescent protein (GFP) asFP499 from Anemonia sulcata is a distant homologue of the GFP from Aequorea victoria. We cloned the asFP499 gene into a mammalian expression vector and showed that this protein was expressed in the human lymphoblast cell line Ramos RA1 and in the embryonic kidney 293T cell line (HEK 293T). In HEK 293T cells, asFP499 was localized mainly in the cytoplasm, suggesting that the protein was excluded from the nucleus. We identified 194LRMEKLNI201 as a candidate nuclear export signal in asFP499 and mutated the isoleucine at position 201 to an alanine. Unlike the wildtype form, the mutant protein was distributed throughout the cytoplasm and nucleus. This is the first report of a GFP that contains a functional NES.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 4, 21 April 2006, Pages 1178–1182
نویسندگان
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