کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940083 1050773 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region
چکیده انگلیسی

The genomic DNA of crucian carp (Carassius cuvieri) metallothionein-II (ccMT-II), with its upstream region, was obtained. The sequence analysis of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (MREs), three activator protein 1 (AP1), two glucocorticoid response elements (GREs), etc. The seven MREs locate into two clusters, a distal cluster with four MREs within −800/−600 bp from the translation start site and a proximal cluster with three MREs close to TATA box. In transient luciferase gene expression assays, both of the distal and proximal cluster MREs have significantly shown synergistic effects in the transcription of ccMT-II gene; the proximal cluster of MREs serves as the major elements in metal inducing activity; Zn2+ and Cd2+ served as much stronger inducers than Cu2+ shown in ccMT-II expression. The two GRE homologous sequences in ccMT-II promoter showed not to be inductive in either HepG2 or HEK293.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 4, 21 April 2006, Pages 1297–1304
نویسندگان
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