کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1940096 | 1050773 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Critical role of cholic acid for development of hypercholesterolemia and gallstones in diabetic mice Critical role of cholic acid for development of hypercholesterolemia and gallstones in diabetic mice](/preview/png/1940096.png)
We studied bile acid and cholesterol metabolism in insulin-dependent diabetes utilizing genetically modified mice unable to synthesize cholic acid (Cyp8b1−/−). Diabetes was induced in Cyp8b1−/− and wild type animals (Cyp8b1+/+) by alloxan, and the mice were fed normal or cholesterol-enriched diet for 10 weeks. The serum levels of cholesterol were strongly increased in diabetic Cyp8b1+/+ mice fed cholesterol, while diabetic Cyp8b1−/− mice did not show any aberrations regardless of the diet. Diabetic cholesterol-fed Cyp8b1+/+ mice had much higher biliary cholesterol and cholesterol saturation index than all other groups, their bile contained a large number of cholesterol crystals, and their canalicular cholesterol transporter Abcg5/g8 mRNA levels were much higher. Cyp7a1 mRNA levels were similar in all diabetic mice but higher compared to non-diabetic animals. The results indicate a critical role for cholic acid for the development of hypercholesterolemia and gallstones in our animal model.
Journal: Biochemical and Biophysical Research Communications - Volume 342, Issue 4, 21 April 2006, Pages 1382–1388