کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940144 1050774 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes
چکیده انگلیسی

Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 346, Issue 1, 21 July 2006, Pages 288–292
نویسندگان
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