کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1940578 | 1050784 | 2006 | 6 صفحه PDF | دانلود رایگان |
It is generally believed that sphingomyelin- and cholesterol-enriched microdomains can be isolated as detergent-resistant membranes (DRMs) from plasma membrane and organelle membranes. Here, we describe the isolation and characterization of microdomains from sarcoplasmic reticulum (SR) membranes. These SR-derived detergent-resistant membranes (SR-DRMs) enriched in sphingomyelin and cholesterol have a low buoyant density. Immunofluorescence microscopy of SR membranes shows the presence of caveolin-3 in the SR, known as a marker protein of caveolae at plasma membrane. We also demonstrated that significant amount of SERCAs together with caveolin-3 associates with SR-DRMs and are fully functional. Depletion of cholesterol caused the disruption of SR-DRMs.
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 4, 16 June 2006, Pages 1135–1140