کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940742 1050787 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intra-strain polymorphisms are detected but no genomic alteration is found in cloned mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Intra-strain polymorphisms are detected but no genomic alteration is found in cloned mice
چکیده انگلیسی

In-gel competitive reassociation (IGCR) is a method for differential subtraction of polymorphic (RFLP) DNA fragments between two DNA samples of interest without probes or specific sequence information. Here, we applied the IGCR procedure to two cloned mice derived from an F1 hybrid of the C57BL/6Cr and DBA/2 strains, in order to investigate the possibility of genomic alteration in the cloned mouse genomes. Each of the five of the genomic alterations we detected between the two cloned mice corresponded to the ‘intra-strain’ polymorphisms in the C57BL/6Cr and DBA/2 mouse strains. Our result suggests that no severe aberration of genome sequences occurs due to somatic cell nuclear transfer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 348, Issue 1, 15 September 2006, Pages 166–169
نویسندگان
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