کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1940937 | 1050791 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein](/preview/png/1940937.png)
چکیده انگلیسی
Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein VH fragment linked with an anti-c-Met VH), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one VH domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 339, Issue 1, 6 January 2006, Pages 305-312
Journal: Biochemical and Biophysical Research Communications - Volume 339, Issue 1, 6 January 2006, Pages 305-312
نویسندگان
Shin-Young Im, Ki Su Kim, Chae-Ok Yun, Joo-Hang Kim, Kye-Sook Yi, Junho Chung,