کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1940950 1050791 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RshA mimetic peptides inhibiting the transcription driven by a Mycobacterium tuberculosis sigma factor SigH
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
RshA mimetic peptides inhibiting the transcription driven by a Mycobacterium tuberculosis sigma factor SigH
چکیده انگلیسی

SigH, an alternative σ factor in Mycobacterium tuberculosis, is a central regulator in responses to the oxidative and heat stress. This SigH activity is specifically controlled by an anti-σ factor RshA during expression of stress-related genes. Thus, the specific interaction (kon = 1.15 × 105 (M−1 s−1), koff = 1.7 × 10−3 (s−1), KD = 15 nM, determined in this study) between SigH and RshA is crucial for the survival and pathogenesis of M. tuberculosis. Using phage-display peptide library, we defined three specific regions on RshA responsible for SigH binding. Three RshA mimetic peptides (DAHADHD, AEVWTLL, and CTPETRE) specifically inhibited the transcription initiated by SigH in vitro. One of them (DAHADHD) diminished the extent of binding of RshA to SigH in a dose-dependent manner. The binding affinity (KD) of this peptide to SigH was about 1.2 μM. These findings might provide some insights into the development of new peptide-based drugs for TB.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 339, Issue 1, 6 January 2006, Pages 392–398
نویسندگان
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