کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941011 1050793 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MIN6 β-cell–β-cell interactions influence insulin secretory responses to nutrients and non-nutrients
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
MIN6 β-cell–β-cell interactions influence insulin secretory responses to nutrients and non-nutrients
چکیده انگلیسی

Insulin-secreting MIN6 cells show greatly enhanced secretory responsiveness to nutrients when grown as islet-like structures (pseudoislets). Since β-cells use different mechanisms to respond to nutrient and non-nutrient stimuli, we have now investigated the role of homotypic β-cell interactions in secretory responses to pharmacological or receptor-operated non-nutrient stimuli in MIN6 pseudoislets. In addition to an enhanced secretory responsiveness to glucose, insulin secretion from MIN6 pseudoislets was also enhanced by non-nutrients, including carbachol, tolbutamide, PMA, and forskolin. The improved secretory responsiveness was dependent on the cells being configured as pseudoislets and was lost on dispersal of the pseudoislets into single cells and regained on the re-formation of pseudoislet structures. These observations emphasise the importance of islet anatomy on secretory responsiveness, and demonstrate that homotypic β-cell interactions play an important role in generating physiologically appropriate insulin secretory responses to both nutrient and non-nutrient stimuli.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 343, Issue 1, 28 April 2006, Pages 99–104
نویسندگان
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