کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941099 1050797 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region
چکیده انگلیسی

Xenopus Zic3 (zinc finger in the cerebellum-3) is expressed in the dorsal neural tube of tailbud embryos and tadpoles. We have isolated a 3.1 kb DNA fragment from the Xenopus laevis Zic3 locus that drives proper expression of a GFP reporter in transgenic frog tailbud embryos and tadpoles. This fragment contains regions that are highly similar among frogs, mice, and humans. One extremely conserved region contains a predicted Pbx binding site. We found that the transcription factors Pbx1b and Meis1 can bind this site and synergistically transactivate expression of a reporter containing the conserved region. Finally, we found that an intact Pbx site is essential for normal Zic3 promoter activity in transgenic frog embryos. Our data strongly suggest that a highly conserved region of the Zic3 promoter functions by direct interaction with Pbx1b and Meis1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 3, 9 June 2006, Pages 1031–1037
نویسندگان
, , , ,