کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1941203 | 1050800 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A Small Ras-like protein Ray/Rab1c modulates the p53-regulating activity of PRPK A Small Ras-like protein Ray/Rab1c modulates the p53-regulating activity of PRPK](/preview/png/1941203.png)
PRPK phosphorylates serine-15 residue of p53 and enhances transcriptional activity. PRPK possesses a bipartite nuclear localization signal and localizes in nucleus when over-expressed in cells. However, intrinsic PRPK localizes mainly in the cytosol in situ. While studying the mechanisms in the distribution of intrinsic PRPK, we identified a PRPK binding protein, an ubiquitously expressed Small Ras-like GTPase, Rab1c, also named Ray or Rab35. The over-expressed Ray was distributed in the nucleus, cytosol, and cell membrane. Both Ray wild type and GTP-restrictively binding mutant Ray-Q67L, but not guanine nucleotide unstable binding mutant Ray-N120I, partially distributed the over-expressed PRPK to the cytosol and also suppressed the PRPK-induced p53-transcriptional activity profoundly. A Small Ras-like GTPase protein Ray was thus indicated to modulate p53 transcriptional activity of PRPK.
Journal: Biochemical and Biophysical Research Communications - Volume 344, Issue 1, 26 May 2006, Pages 377–385