کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941220 1050805 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A G protein γ subunit peptide stabilizes a novel muscarinic receptor state
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A G protein γ subunit peptide stabilizes a novel muscarinic receptor state
چکیده انگلیسی

A prenylated peptide specific to the C terminal tail of a G protein γ subunit type, γ5, inhibits activation of a G protein by the M2 muscarinic receptor. The γ5 peptide was tested for direct effects on the M2 receptor’s properties. The wild type γ5 peptide reduced the affinity of M2 for the agonist, carbachol, more than 5-fold in an antagonist displacement assay. The peptide was inactive when its amino acid sequence was scrambled or when it was unprenylated. Although the wild type peptide reduced the affinity of M2 for the antagonist QNB, it had no effect on the antagonists NMS or atropine. These results suggest that in the presence of the peptide the M2 receptor adopts a novel conformational state that affects the ligand binding surface. The results also suggest that the G protein γ5 subunit tail interacts with a receptor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 341, Issue 4, 24 March 2006, Pages 904–910
نویسندگان
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