کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1941315 1050808 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glucose regulated proteins 78 and 75 bind to the receptor for hyaluronan mediated motility in interphase microtubules
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Glucose regulated proteins 78 and 75 bind to the receptor for hyaluronan mediated motility in interphase microtubules
چکیده انگلیسی

The receptor for hyaluronan mediated motility (RHAMM), which is a hyaluronan-binding protein, is a centrosomal and microtubal protein. Here, we have identified two RHAMM-binding proteins, glucose regulated protein (GRP) 78 and GRP75, using co-immunoprecipitation analysis. These two proteins directly bound to glutathione-S-transferase-RHAMM fusion proteins. By double immunostaining, GRP78 and GRP75 colocalized with RHAMM in interphase microtubules, but were separated in mitotic spindles. Prevention of microtubule polymerization by TN-16 and vincristine sulfate induced RHAMM overexpression without a significant change in GRP78/75. Taken together, GRP78/75 and RHAMM complexes may stabilize microtubules in the interphase, associated with a downregulation of RHAMM. These results reveal a new biochemical activity of RHAMM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 339, Issue 3, 20 January 2006, Pages 971–976
نویسندگان
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