کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1941929 | 1052560 | 2016 | 9 صفحه PDF | دانلود رایگان |
• Oxidation of tyrosine-Z triggers substrate water reorganization in PSII.
• A calcium-bound water molecule moves upon deprotonation to a Mn center.
• W3 and O5 function as substrate waters in the O―O bond formation process.
• O2 formation triggers reorganization of a new Ca-bound water for the next cycle.
• The calcium ion may functions as a substrate water access gate in PSII.
Photosystem II (PSII) catalyzes light-driven water splitting in nature and is the key enzyme for energy input into the biosphere. Important details of its mechanism are not well understood. In order to understand the mechanism of water splitting, we perform here large-scale density functional theory (DFT) calculations on the active site of PSII in different oxidation, spin and ligand states. Prior to formation of the O-O bond, we find that all manganese atoms are oxidized to Mn(IV) in the S3 state, consistent with earlier studies. We find here, however, that the formation of the S3 state is coupled to the movement of a calcium-bound hydroxide (W3) from the Ca to a Mn (Mn1 or Mn4) in a process that is triggered by the formation of a tyrosyl radical (Tyr-161) and its protonated base, His-190. We find that subsequent oxidation and deprotonation of this hydroxide on Mn1 result in formation of an oxyl-radical that can exergonically couple with one of the oxo-bridges (O5), forming an O-O bond. When O2 leaves the active site, a second Ca-bound water molecule reorients to bridge the gap between the manganese ions Mn1 and Mn4, forming a new oxo-bridge for the next reaction cycle. Our findings are consistent with experimental data, and suggest that the calcium ion may control substrate water access to the water oxidation sites.
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1857, Issue 6, June 2016, Pages 740–748