کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1942419 | 1052610 | 2012 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa Phycobilisome model with novel skeleton-like structures in a glaucocystophyte Cyanophora paradoxa](/preview/png/1942419.png)
Phycobilisome (PBS) is a photosynthetic antenna supercomplex consisting of a central core subcomplex with several peripheral rods radiating from the core. Subunit structure of PBS was studied in a glaucocystophyte Cyanophora paradoxa strain NIES 547. Subunit composition of PBS was identified by N-terminal sequencing and genes for the subunits were determined by homology search of databases. They included rod linker proteins CpcK1 and CpcK2, rod-core linker proteins CpcG1 and CpcG2, and core linker proteins ApcC1 and ApcC2. Subfractionation by native polyacrylamide gel electrophoresis provided evidence for novel subcomplexes (ApcE/CpcK1/CpcG2/ApcA/ApcB/CpcD and ApcE/CpcK2/CpcG1/ApcA/ApcB), which connect rod and core subcomplexes. These skeleton-like structures may serve as a scaffold of the whole PBS assembly. Different roles of ApcC1 and ApcC2 were also suggested. Based on these findings, structural models for PBS were proposed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.
► Novel skeleton-like scaffold for assembly of phycobilisome supercomplex.
► Novel rod linker proteins CpcK1 and CpcK2 with tandem repeat liker domains.
► Two distinct subpopulations of phycobilisome.
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1817, Issue 8, August 2012, Pages 1428–1435