کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1942457 1052614 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyamines induce aggregation of LHC II and quenching of fluorescence in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Polyamines induce aggregation of LHC II and quenching of fluorescence in vitro
چکیده انگلیسی

Dissipation of excess excitation energy within the light-harvesting complex of Photosystem II (LHC II) is a main process in plants, which is measured as the non-photochemical quenching of chlorophyll fluorescence or qE. We showed in previous works that polyamines stimulate qE in higher plants in vivo and in eukaryotic algae in vitro. In the present contribution we have tested whether polyamines can stimulate quenching in trimeric LHC II and monomeric light-harvesting complex b proteins from higher plants. The tetramine spermine was the most potent quencher and induced aggregation of LHC II trimers, due to its highly cationic character. Two transients are evident at 100 μM and 350 μM for the fluorescence and absorbance signals of LHC II respectively. On the basis of observations within this work, some links between polyamines and the activation of qE in vivo is discussed.


► Polyamines quench Chl fluorescence in monomeric Lhcb proteins and trimeric LHC II.
► The relative rank of effectiveness is Spm > Spd > Put.
► Spermine induces aggregation of trimeric LHC II.
► Two transients are evident for the fluorescence and absorbance signals of LHC II.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1817, Issue 5, May 2012, Pages 735–743
نویسندگان
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