کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944296 1053203 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Neutral liposomes containing crown ether-lipids as potential DNA vectors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Neutral liposomes containing crown ether-lipids as potential DNA vectors
چکیده انگلیسی


• Three crown ether derivatives have been inserted into neutral liposomes.
• The effects of the guests on the bilayer have been studied by different techniques.
• Kinetic and thermodynamic profiles of mixed liposomes have been studied.
• In the presence of Ca2 + the crown ether lipids induce the DNA complexation.

Three crown ether derivatives, 1,2-O-dioleoyl-3-O-{2-[(12-crown-4)methoxy]ethyl}-sn-glycerol (12C4L), 1,2-O-dioleoyl-3-O-{2-[(15-crown-5)methoxy]ethyl}-sn-glycerol (15C5L) and 2,3-naphtho-15-crown-5 (NAP5), have been incorporated into 1-palmitoyl-2-oleoyl-phosphatydilcholine (POPC) liposomes. The size of the crown ether and the lipophilic moiety of 12C4L, 15C5L and NAP5 influence the stability and the properties of the extruded POPC liposomes determined at 25 °C in buffered aqueous solution at pH 7.4. The investigated liposomes are zwitterionic for POPC headgroups but can be turned into cationic aggregates in the presence of divalent cations. The capability of these systems to complex DNA has been demonstrated by SAXS experiments.

Figure optionsDownload high-quality image (271 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1828, Issue 11, November 2013, Pages 2506–2512
نویسندگان
, , , , ,