کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1944308 | 1053203 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The structure of the CD3 ζζ transmembrane dimer in POPC and raft-like lipid bilayer: A molecular dynamics study The structure of the CD3 ζζ transmembrane dimer in POPC and raft-like lipid bilayer: A molecular dynamics study](/preview/png/1944308.png)
• We examine the effect of lipids on structure and dynamics of the CD3 ζζ dimer.
• Waters contribute to geometry of Asp–Asp site, critical for binding to the receptor.
• Critical interactions between ζζ and lipids occur at lipid–cytoplasm boundary.
Plasma membrane lipids significantly affect assembly and activity of many signaling networks. The present work is aimed at analyzing, by molecular dynamics simulations, the structure and dynamics of the CD3 ζζ dimer in palmitoyl-oleoyl-phosphatidylcholine bilayer (POPC) and in POPC/cholesterol/sphingomyelin bilayer, which resembles the raft membrane microdomain supposed to be the site of the signal transducing machinery. Both POPC and raft-like environment produce significant alterations in structure and flexibility of the CD3 ζζ with respect to nuclear magnetic resonance (NMR) model: the dimer is more compact, its secondary structure is slightly less ordered, the arrangement of the Asp6 pair, which is important for binding to the Arg residue in the alpha chain of the T cell receptor (TCR), is stabilized by water molecules. Different interactions of charged residues with lipids at the lipid–cytoplasm boundary occur when the two environments are compared. Furthermore, in contrast to what is observed in POPC, in the raft-like environment correlated motions between transmembrane and cytoplasmic regions are observed. Altogether the data suggest that when the TCR complex resides in the raft domains, the CD3 ζζ dimer assumes a specific conformation probably necessary to the correct signal transduction.
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Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1828, Issue 11, November 2013, Pages 2637–2645