کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944546 1053221 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transmembrane helices can induce domain formation in crowded model membranes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Transmembrane helices can induce domain formation in crowded model membranes
چکیده انگلیسی

We studied compositionally heterogeneous multi-component model membranes comprised of saturated lipids, unsaturated lipids, cholesterol, and α-helical TM protein models using coarse-grained molecular dynamics simulations. Reducing the mismatch between the length of the saturated and unsaturated lipid tails reduced the driving force for segregation into liquid-ordered (lo) and liquid-disordered (ld) lipid domains. Cholesterol depletion had a similar effect, and binary lipid mixtures without cholesterol did not undergo large-scale phase separation under the simulation conditions. The phase-separating ternary dipalmitoyl-phosphatidylcholine (DPPC)/dilinoleoyl-PC (DLiPC)/cholesterol bilayer was found to segregate into lo and ld domains also in the presence of a high concentration of ΤΜ helices. The ld domain was highly crowded with TM helices (protein-to-lipid ratio ~ 1:5), slowing down lateral diffusion by a factor of 5–10 as compared to the dilute case, with anomalous (sub)-diffusion on the μs time scale. The membrane with the less strongly unsaturated palmitoyl-linoleoyl-PC instead of DLiPC, which in the absence of TM α-helices less strongly deviated from ideal mixing, could be brought closer to a miscibility critical point by introducing a high concentration of TM helices. Finally, the 7-TM protein bacteriorhodopsin was found to partition into the ld domains irrespective of hydrophobic matching. These results show that it is possible to directly study the lateral reorganization of lipids and proteins in compositionally heterogeneous and crowded model biomembranes with coarse-grained molecular dynamics simulations, a step toward simulations of realistic, compositionally complex cellular membranes. This article is part of a Special Issue entitled: Protein Folding in Membranes.

Figure optionsDownload high-quality image (525 K)Download as PowerPoint slideHighlights
► Saturated/unsaturated lipid/cholesterol mixtures can segregate in MD simulations.
► A high concentration of TM helices in the membrane slows down diffusion.
► TM helices can amplify non-ideal lipid mixing.
► Bacteriorhodopsin partitions into liquid-disordered lipid domains.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1818, Issue 4, April 2012, Pages 984–994
نویسندگان
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