کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944621 1053231 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of a bacterial trehalose lipid on phosphatidylglycerol membranes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effects of a bacterial trehalose lipid on phosphatidylglycerol membranes
چکیده انگلیسی

Bacterial trehalose lipids are biosurfactants with potential application in the biomedical/healthcare industry due to their interesting biological properties. Given the amphiphilic nature of trehalose lipids, the understanding of the molecular mechanism of their biological action requires that the interaction between biosurfactant and membranes is known. In this study we examine the interactions between a trehalose lipid from Rhodococcus sp. and dimyristoylphosphatidylglycerol membranes by means of differential scanning calorimetry, X-ray diffraction, infrared spectroscopy and fluorescence polarization. We report that there are extensive interactions between trehalose lipid and dimyristoylphosphatidylglycerol involving the perturbation of the thermotropic gel to liquid-crystalline phase transition of the phospholipid, the increase of fluidity of the phosphatidylglycerol acyl chains and dehydration of the interfacial region of the bilayer, and the modulation of the order of the phospholipid bilayer. The observations are interpreted in terms of structural perturbations affecting the function of the membrane that might underline the biological actions of the trehalose lipid.

Research highlights
► Trehalose lipid perturbs the phase transition of dimyristoylphosphatidylglycerol.
► The biosurfactant increases the fluidity of the phosphatidylglycerol acyl chains.
► Trehalose lipid dehydrates the interfacial region of the bilayer.
► Trehalose lipid modulates the order of the phospholipid palisade.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1808, Issue 8, August 2011, Pages 2067–2072
نویسندگان
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