کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1944622 1053231 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of the putative N-glycosylation and PKC-phosphorylation sites of the human sodium-dependent multivitamin transporter (hSMVT) in function and regulation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Role of the putative N-glycosylation and PKC-phosphorylation sites of the human sodium-dependent multivitamin transporter (hSMVT) in function and regulation
چکیده انگلیسی

The sodium-dependent multivitamin transporter (SMVT) is a major biotin transporter in a variety of tissues including the small intestine. The human SMVT (hSMVT) polypeptide is predicted to have four N-glycosylation sites and two putative PKC phosphorylation sites but their role in the function and regulation of the protein is not known and was examined in this investigation. Our results showed that the hSMVT protein is glycosylated and that this glycosylation is important for its function. Studies utilizing site-directed mutagenesis revealed that the N-glycosylation sites at positions Asn138 and Asn489 are important for the function of hSMVT and that mutating these sites significantly reduces the Vmax of the biotin uptake process. Mutating the putative PKC phosphorylation site Thr286 of hSMVT led to a significant decrease in the PMA-induced inhibition in biotin uptake. The latter effect was not mediated via changes in the level of expression of the hSMVT protein and mRNA or in its level of expression at the cell membrane. These findings demonstrate that the hSMVT protein is glycosylated, and that glycosylation is important for its function. Furthermore, the study shows a role for the putative PKC-phosphorylation site Thr286 of hSMVT in the PKC-mediated regulation of biotin uptake.


► The human sodium-dependent multivitamin transporter (hSMVT) is glycosylated.
► The glycosylation Asn138 and Asn489 are essential for hSMVT function.
► The putative PKC-phosphorylation site Thr286 in the hSMVT polypeptide is important in the PKC-mediated regulation of biotin uptake.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1808, Issue 8, August 2011, Pages 2073–2080
نویسندگان
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