کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1946694 1537284 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Juvenile hormone binding protein core promoter is TATA-driven with a suppressory element
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Juvenile hormone binding protein core promoter is TATA-driven with a suppressory element
چکیده انگلیسی

The hemolymph juvenile hormone binding protein (JHBP) plays a key role in transporting juvenile hormone (JH) to target tissues and in protecting JH from the degradation by nonspecific esterases. Juvenile hormone esterase (JHE) removes JH signal at precisely defined insect developmental stages. The functional analysis of regulatory elements in the core promoter has been described only for the jhe gene. In this report we define the core promoter functional elements in the Galleria mellonella jhbp gene. It appears that jhbp core promoter is under strong control of TATA box and the transcription site (tss). In contrast to regulation of the jhe gene, the jhbp core promoter contains a sequence which directly suppresses jhbp expression. Evidences are provided for the contribution of the will die slowly (WDS) suppressory protein in jhbp basal transcription. We have also shown that the activity of the jhbp core promoter can be regulated by JHIII, and lesser so by 20E.

Research Highlight
► Jhbp core promoter is TATA box driven and suppressed by will die slowly protein.
► Core DPE-like element participates in suppressing jhbp gene expression.
► Tss and Inr-like elements play an opposite effect in jhbp gene control.
► In contrast to jhbp the jhe core promoter is controlled by activatory elements only

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1809, Issues 4–6, April–June 2011, Pages 226–235
نویسندگان
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