کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1948302 1054685 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A mannose/glucose-specific lectin from Chinese evergreen chinkapin (Castanopsis chinensis)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A mannose/glucose-specific lectin from Chinese evergreen chinkapin (Castanopsis chinensis)
چکیده انگلیسی

A mannose/glucose-specific lectin has been purified from Chinese evergreen chinkapin (Castanopsis chinensis) seeds, one of the most popular foods in East Asia. This lectin, designated as CCL, exhibited hemagglutinating activity in mouse and rabbit erythrocytes. It displayed a single band with a molecular mass of 29 kDa in SDS-PAGE and a 120-kDa peak in gel-filtration on Superdex-200. Its hemagglutinating activity was stable in the pH range 6–12 and at temperatures below 60 °C. The N-terminal amino acid sequence of CCL differed from those of other lectins in the same family. CCL inhibited the proliferation of HepG2 cells and adult emergence in fruitflies. CCL exhibited mitogenic activity toward mouse splenocytes, and induced nitric oxide production from mouse peritoneal macrophages but was devoid of inhibitory activity toward mycelial growth and HIV-1 reverse transcriptase.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1780, Issue 9, September 2008, Pages 1017–1022
نویسندگان
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