کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1948514 1054699 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Identification of palmitate-regulated genes in HepG2 cells by applying microarray analysis
چکیده انگلیسی

Palmitate is the most abundant saturated fatty acid in the human diet and the major one synthesized de novo. To identify palmitate-regulated genes we performed whole genome mRNA expression profiling by using human hepatoma HepG2 cells. We identified eleven genes which are significantly (single-sided permutational t-test, p < 0.05) regulated by low concentration of palmitate (50 μM). We observed a decreased expression of five metallothioneins, and an increased expression of liver expressed plasminogen activator inhibitor-1 protein and insulin-like growth factor II, which play a prominent role in the development of the metabolic syndrome. Comparative promoter analysis in-silico revealed common transcriptional regulation of differentially expressed genes through erythroid kruppel-like factor and members of the zinc binding protein factor family. In conclusion, low physiological palmitate concentrations changed expression of very responsive genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 9, September 2007, Pages 1283–1288
نویسندگان
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