کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1948528 | 1054699 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of Ca2+/calmodulin-dependent protein kinases in mycelial growth of the basidiomycetous mushroom, Coprinus cinereus
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کلمات کلیدی
Coprinus cinereusCaM-kinaseBasidiomycete - basidiomyceteSer/Thr kinase - Ser / Thr کینازCAM - ساخت به کمک کامپیوترProtein phosphorylation - فسفوریلاسیون پروتئینMushroom - قارچ یا ماشرومprotein serine/threonine kinase - پروتئین serine / ترئونین کینازCa2+/calmodulin-dependent protein kinase - پروتئین کیناز وابسته به کلسیم و پتاسیم Ca2 + /Calmodulin - کالمودولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Involvement of Ca2+/calmodulin-dependent protein kinases in mycelial growth of the basidiomycetous mushroom, Coprinus cinereus Involvement of Ca2+/calmodulin-dependent protein kinases in mycelial growth of the basidiomycetous mushroom, Coprinus cinereus](/preview/png/1948528.png)
چکیده انگلیسی
Although multifunctional Ca2+/calmodulin-dependent protein kinases (CaM-kinases) are widely distributed in animal cells, the occurrence of CaM-kinases in the basidiomycetous mushroom has not previously been documented. When the extracts from various developmental stages from mycelia to the mature fruiting body of Coprinus cinereus were analyzed by Western blotting using Multi-PK antibodies, which had been generated to detect a wide variety of protein serine/threonine kinases (Ser/Thr kinases), a variety of stage-specific Ser/Thr kinases was detected. Calmodulin (CaM) overlay assay using digoxigenin-labeled CaM detected protein bands of 65 kDa, 58 kDa, 46 kDa, 42 kDa, and 38 kDa only in the presence of CaCl2, suggesting that these bands were CaM-binding proteins. When the CaM-binding fraction was prepared from mycelial extract of C. cinereus by CaM-Sepharose and analyzed with Multi-PK antibodies, two major immunoreactive bands corresponding to 65Â kDa and 46Â kDa were detected. CaM-binding fraction, thus obtained, exhibited Ca2+/CaM-dependent protein kinase activity toward protein substrates such as histones. These CaM-kinases were found to be highly expressed in the actively growing mycelia, but not in the resting mycelial cells. Mycelial growth was enhanced by the addition of CaCl2 in the culture media, but inhibited by the addition of EGTA or trifluoperazine, a potent CaM inhibitor. This suggested that CaM-dependent enzymes including CaM-kinases play crucial roles in mycelial growth of basidiomycete C. cinereus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 9, September 2007, Pages 1395-1403
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 9, September 2007, Pages 1395-1403
نویسندگان
Isamu Kameshita, Yusuke Yamada, Tetsuyuki Nishida, Yasunori Sugiyama, Noriyuki Sueyoshi, Akira Watanabe, Yasuhiko Asada,