کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1948661 | 1054705 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Complex oligosaccharides are N-linked to Kv3 voltage-gated K+ channels in rat brain
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کلمات کلیدی
CDGPNGase FK+ channel - K + کانالcongenital disorder of glycosylation - اختلال مادرزادی گلیسوزیله شدنCongenital disorders of glycosylation - اختلالات مادرزادی گلیکوزیلاتEndo H - اندو هendoglycosidase H - اندوگلیکوزیداز HTopology - توپولوژیBrain - مغزVoltage-gated K+ channel - کانال K + با ولتاژGlycosylation - گلیکوزیله شدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Neuronal Kv3 voltage-gated K+ channels have two absolutely conserved N-glycosylation sites. Here, it is shown that Kv3.1, 3.3, and 3.4 channels are N-glycosylated in rat brain. Digestion of total brain membranes with peptide N glycosidase F (PNGase F) produced faster migrating immunobands than those of undigested membranes. Additionally, partial PNGase F digests showed that both sites are occupied by oligosaccharides. Neuraminidase treatment produced a smaller immunoband shift relative to PNGase F treatment. These results indicate that both sites are highly available and occupied by N-linked oligosaccharides for Kv3.1, 3.3, and 3.4 in rat brain, and furthermore that at least one oligosaccharide is of complex type. Additionally, these results point to an extracytoplasmic S1-S2 linker in Kv3 proteins expressed in native membranes. We suggest that N-glycosylation processing of Kv3 channels is critical for the expression of K+ currents at the surface of neurons, and perhaps contributes to the pathophysiology of congenital disorders of glycosylation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 4, April 2007, Pages 666-671
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1770, Issue 4, April 2007, Pages 666-671
نویسندگان
Tara A. Cartwright, Melissa J. Corey, Ruth A. Schwalbe,