Keywords: PNGase F; peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine-amidase; hIgG1; Immunoglobulin G1; APTS; 8-aminopyrene-1,3,6-trisulfonic acid; THF; tetrahydrofuran; Evaporation; Fluorophore labeling; APTS; Capillary electrophoresis;
مقالات ISI (ترجمه نشده)
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Keywords: 2-AA; 2-aminobenzoic acid; ABC; ammonium bicarbonate; ACN; acetonitrile; DHB; 2,5-dihydroxybenzoic acid; DTT; dithiothreitol; FA; formic acid; HILIC-FLD; hydrophilic interaction liquid chromatography-fluorescence detection; IAA; iodacetamide; MALDI; matri
Characterization of drug load variants in a thiol linked antibody-drug conjugate using multidimensional chromatography
Keywords: ADC; antibody-drug conjugate; MS; mass spectrometry; PNGase F; peptide-N-glycosidase F; QTOF; quantitative time of flight; UV; ultraviolet; RP; reversed-phase liquid chromatography; nSEC; native size exclusion chromatography; nrRP; non-reduced reversed-ph
Keywords: PNGase F; Peptide -N-Glycosidase F; endo H; endoglycosidase H; ER; endoplasmic reticulum; LPH; lactase-phlorizin hydrolase (all forms); LPHh; mannose-rich precursor; LPHc; complex glycosylated precursor; TGN; trans-Golgi network; Human intestinal lactase-
Keywords: AGE; Advanced Glycation End Products; ddPCR; droplet digital PCR; DHA; docosahexaenoic acid; EC; endothelial cells; eNOS; endothelial Nitric Oxide Synthase; GLUT; glucose transporter; HAoEC; Human Aortic Endothelial Cells; HDMEC; Human Dermal Microendothe
Keywords: AKH; adipokinetic hormone; CHO; Chinese hamster ovary; Crypα; cell adhesion molecule-like tyrosine phosphatase α; Endo H; Endoglycosidase H; ENG; endoglin/CD105; HEK; human embryonic kidney; IMAC; immobilized metal affinity chromatography; MBP; maltose-
Keywords: ALP; alkaline phosphatase; ER; endoplasmic reticulum; Endo H; endo-β-N-acetylglucosaminidase H; DOX; doxycycline; GPI; glycosylphosphatidylinositol; HPP; hypophosphatasia; PI-PLC; phosphatidylinositol-specific phospholipase C; PNGase F; peptide-N-glycosi
Keywords: IgG; immunoglobulin G; ATP; adenosine triphosphate; NAD+; nicotinamide adenine dinucleotide; CNBr; cyanogens bromide; PNGase F; peptide N-glycosidase F; BHI; Brain-Heart Infusion; ANTS; 8-aminonaphthalene-1,3,6-trisulfonate; BCIP; 5-Bromo 4-Chloro 3â²
Keywords: mAb; monoclonal antibody; IgG; immunoglobulin G; HPAEC-PAD; high-pH anion-exchange chromatography with pulsed amperometric detection; HILIC-FD; hydrophilic-interaction chromatography with fluorescence detection; 2-AA; 2-aminobenzoic acid (anthranilic acid
Keywords: 39K; baculovirus delayed early gene encoding phosphoprotein of 39 kDa in apparent molecular weight; AmBic; ammonium bicarbonate buffer; BCA; bicinchoninic acid; B4GALT1β; 1,4-galactosyltransferase I; BEVS; baculovirus expression vector system; CMAS; CMP
Keywords: 5-FU TM; 5-fluorouracil tunicamycin; PBS; phosphate-buffered saline; PBST; PBS containing 0.1% Tween 20; TBS; tris buffered saline; DMSO; dimethyl sulfoxide; PNGase F; peptide N glycosidase F; IR; tumor inhibition rate; Glycogene; N-glycan; Invasion; Chem
Expression of glycogenes in differentiating human NT2N neurons. Downregulation of fucosyltransferase 9 leads to decreased Lewisx levels and impaired neurite outgrowth
Keywords: BSA; bovine serum albumin; dHex; deoxyhexose; EDTA; etylenediaminetetraacetic acid; EGTA; ethylene glycol tetraacetic acid; Fuc; fucose; Gal; galactose; GlcNAc; N-acetylglucosamine; Hex; hexose; HexNAc; N-acetylhexosamine; HNK-1; human natural killer-1; H
Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis
Keywords: AETMA; (2 aminoethyl)trimethylammonium chloride; DP3; isomaltotriose; DP4; maltotetraose; DP5; maltopentaose; DP6; maltohexaose; DP7; maltoheptaose; PNGase F; peptide-N-glycosidase F; RNase B; ribonuclease B; Capillary electrophoresis; Hexahistidine; Labe
Investigation of a side reaction occurring during N-linked glycan labeling by cationic tags
Keywords: 2-AA; 2-aminobenzoic acid; AETMA; (2-aminoethyl)trimethylammonium chloride hydrochloride; DP3; isomaltotriose; DP4; maltotetraose; DP5; maltopentaose; DP6; maltohexaose; DP7; maltoheptaose; GT; (hydrazinocarbonylmethyl)trimethylammonium chloride (Girardâ
Secretion and function of Cln5 during the early stages of Dictyostelium development
Keywords: Acb; acyl-coenzyme-binding protein; BFA; brefeldin A; bsr; blasticidin resistance; cAMP; cyclic adenosine monophosphate; CTS; cathepsin; CLN; ceroid lipofuscinosis neuronal; CM; conditioned media; CMF; conditioned media factor; CV; contractile vacuole; CQ
Comparison of anti-pathogenic activities of the human and bovine milk N-glycome: Fucosylation is a key factor
Keywords: bLF; bovine Lactoferrin; hLF; human Lactoferrin; PNGase F; peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidase from Flavobacterium meningosepticum; MOS; milk oligosaccharides; 2AB; 2-aminobenzamide; PGC; nonporous graphitized carbon; HILIC-UHPLC; hyd
Biochemical, biological and molecular characterization of an L-Amino acid oxidase (LAAO) purified from Bothrops pictus Peruvian snake venom
Keywords: L-amino acid oxidase; Bothrops pictus; Bpic-LAAO; Bpic-LAAO; Bothrops pictus-L-amino acid oxidase; CFU; colony-forming units; FAD; flavin adenine dinucleotide; LAAO; L-amino acid oxidase; PNGase F; peptide N-glucosidase; RP-HPLC; reverse-phase high perfor
Hepatic ABCA1 deficiency is associated with delayed apolipoprotein B secretory trafficking and augmented VLDL triglyceride secretion
Keywords: ABCA1; ATP binding cassette transporter A1; TG; triglyceride; HSKO; hepatocyte-specific ABCA1 knockout; apoB; apolipoprotein B; McA; rat hepatoma McA RH7777 cells; Endo H; endoglycosidase H; PNGase F; peptide N-glycosidase F2; MTP; microsomal triglyceride
N-Glycoform-dependent interactions of megalin with its ligands
Keywords: DBP; vitamin D-binding protein; Fut8; α1,6-fucosyltransferase; JCGGDB; Japan Consortium for Glycobiology and Glycotechnology Database; Kan; kanamycin; LCA; Lens culinaris agglutinin; LRP; LDL receptor-related protein; MAS; Matsunami Adhesive Silane; NP-4
Rapid N-glycan release from glycoproteins using immobilized PNGase F microcolumns
Keywords: PNGase F; Peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine-amidase; GST; glutathione-S-transferase; RNase B; ribonuclease B; IgG; immunoglobulin G; APTS; 8-aminopyrene-1,3,6-trisulfonic acid; CGE-LIF; capillary gel electrophoresis with laser induced flu
Nicotiana benthamiana cathepsin B displays distinct enzymatic features which differ from its human relative and aleurain-like protease
Keywords: Antibody; Cathepsin; Cysteine protease; Expression platform; Plant; Proteolysis; Abz; 2-aminobenzoyl; ALP; aleurain-like protease; CathB; cathepsin B; CHN2; diazomethyl; CDR; complementarity-determining region; DTT; dithiothreitol; Endo H; endoglucosamini
Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling
Keywords: Ubiquitin; Innate immunity; TNF; TLR3; NOD1; LUBAC; AMSH-LP; AMSH-like protein; λ-PPase; bacteriophage λ protein phosphatase; BMDM; bone marrow-derived macrophages; JNK; c-Jun N-terminal kinase; cIAP; cellular Inhibitor of Apoptosis; DUB; deubiquitylase
Shoc2-tranduced ERK1/2 motility signals - Novel insights from functional genomics
Keywords: EGF; epidermal growth factor; ERK1/2; extracellular signal-regulated kinase 1 and 2; KSR1; kinase suppressor of Ras (KSR1); MP1; mitogen-activated protein 1; PNGase F; Peptide-N-Glycosidase F; Endo H; Endoglycosidase H; PP1c; protein phosphatase 1c; qPCR;
N-glycosylation profiling of porcine reproductive and respiratory syndrome virus envelope glycoprotein 5
Keywords: PRRSV; porcine reproductive and respiratory syndrome virus; N-glycan; asparagine-linked glycan; PNGase F; peptide-N-glycosidase F; Endo Hf; endoglycosidase Hf; GlcNAc; N-acetylglucosamine; LacNAc; N-acetyllactosamine; Gal; galactose; NeuAc; N-acetylneuram
N-linked glycosylation of the superoxide-producing NADPH oxidase Nox1
Keywords: Nox; NADPH oxidase; ROS; reactive oxygen species; Duox; dual oxidase; ER; endoplasmic reticulum; PNGase F; peptide: N-glycosidase F; Endo H; endoglycosidase H; Nox1; Glycosylation; p22phox; Superoxide;
Development of in vitro model of insulin receptor cleavage induced by high glucose in HepG2 cells
Keywords: ADAM; a disintegrin and metalloprotease; BAPTA-AM; O,Oâ²-Bis [2-aminophenyl] ethyleneglycol-N,N,Nâ²,Nâ²-tetraacetic acid tetraacetoxymethyl ester; DON; deoxynorleucine; ICT-EIA; immune complex transfer enzyme immunoassay; IR; insulin receptor; OGA; β-
N-Glycosylation during translation is essential for human arylacetamide deacetylase enzyme activity
Keywords: AADAC; arylacetamide deacetylase; CES; carboxylesterase; DMSO; dimethyl sulfoxide; Endo H; endoglycosidase H; ER; endoplasmic reticulum; α-Glc; α-glucosidase; GPT; GlcN-Ac-1-phosphate transferase; HLM; human liver microsomes; PNGase F; peptide: N-glycos
Drosophila melanogaster cellular repressor of E1A-stimulated genes is a lysosomal protein essential for fly development
Keywords: CREG; Cellular repressor of E1A-stimulated genes; dCREG; D. melanogaster CREG; GFP; Green fluorescent protein; LERP; Lysosomal enzyme receptor protein; M6P; Mannose 6-phosphate; msCREG; Mouse CREG; PNGase F; Peptide N-glycosidase F; Cathepsin; CREG; Lysos
Deglycosylation systematically improves N-glycoprotein identification in liquid chromatography–tandem mass spectrometry proteomics for analysis of cell wall stress responses in Saccharomyces cerevisiae lacking Alg3p
Keywords: OTase, oligosaccharyltransferaseALG3; N-glycosylation; Saccharomyces cerevisiae; PNGase F; Deglycosylation; Proteomics
Oriented immobilization of peptide-N-glycosidase F on a monolithic support for glycosylation analysis
Keywords: Enzyme microreactor; Oriented immobilization; Monolith; PNGase F; Deglycosylation
Determination of cathepsin V activity and intracellular trafficking by N-glycosylation
Keywords: API; Achromobacter protease I; CBB; Coomassie Brilliant Blue; DMEM; Dulbecco's modified Eagle's medium; ER; endoplasmic reticulum; MALDI-TOF MS; matrix-assisted laser desorption ionization time of flight mass spectrometry; PBS; phosphate-bufferd salin
Glycomic alterations are associated with multidrug resistance in human leukemia
Keywords: MDR; multidrug resistance; ADR; adriamycin; TM; tunicamycin; PBS; phosphate-buffered saline; PBST; PBS containing 0.1% Tween 20; TBS; tris buffered saline; FITC; fluorescein isothiocyanate; MALDI-TOF MS; matrix-assisted laser desorption/ionization time of
Proteolytic cleavage of the disease-related lysosomal membrane glycoprotein CLN7
Keywords: BFA; brefeldin A; CHX; cycloheximide; Ctsb; cathepsin B; Ctsl; cathepsin L; DMEM; Dulbecco's minimal essential medium; endo H; endoglycosidase H; ER; endoplasmic reticulum; GFP; green fluorescent protein; HEK293; human embryonic kidney 293; HGSNAT; hepari
Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells
Keywords: HEK; human embryonic kidney cells; CHO; Chinese hamster ovary cells; TCA; trichloroacetic acid; PEI; polyethylenimine; IEF; isoelectric focusing; PNGase F; peptide N-glycosidase F; APTS; 8-amino-1,3,6-pyrenestisulfonic acid; Glycobiology; Glycoprotein; Is
N- and O-linked glycosylation of total plasma glycoproteins in galactosemia
Keywords: AMH; anti-Müllerian hormone; DHB; 2, 5-dihydroxybenzoic acid; DMSO; dimethyl sulfoxide; GALE; UDP-galactose 4â²-epimerase; GALT; galactose-1-phosphate uridylyltransferase; UDP-Gal; uridine diphosphate galactose; UDP-GalNAc; uridine diphosphate-N-acetyl-
Proteinase 3 carries small unusual carbohydrates and associates with αlpha-defensins
Keywords: PR3; Proteinase 3; NHP; human neutrophil peptide; WG; Wegener's granulomatosis; PMN; polymorphonuclear leukocytes; PAR-2; protease-activated receptor-2; IFN-γ; interferon gamma; ANCA; anti-neutrophil cytoplasmic antibodies; MPO; myeloperoxidase; SAP; ser
Large-scale assignment of N-glycosylation sites using complementary enzymatic deglycosylation
Keywords: Glycoproteomics; Lectin affinity chromatography; PNGase F; Endo H; Mass spectrometry;
Methods development for analysis of partially deglycosylated proteins and application to an HIV envelope protein vaccine candidate
Keywords: Glycosylation; PNGase F; Endoglycosidase; HIV; Envelope protein; Glycosylation site
Identification and characterization of the immunogenic cytotoxic TvCP39 proteinase gene of Trichomonas vaginalis
Keywords: α-His; anti-histidine tag antibodies; α-TvCP39r; anti-recombinant TvCP39 protein antibodies; α-TvCP39n; anti-native TvCP39 antibodies; α-T.v; anti-total trichomonad extract antibodies; BSA; bovine serum albumin; CBB; Coomassie brilliant blue; CNCD; Ce
Production and N-glycosylation of recombinant human cell adhesion molecule L1 from insect cells using the stable expression system. Effect of dimethyl sulfoxide
Keywords: dHex; deoxyhexose; DMSO; dimethyl sulfoxide; EDTA; etylenediaminetetraacetic acid; Endo H; Endo-β-N-acetylglucosaminidase H; Fuc; fucose; Gal; galactose; GlcNAc; N-acetylglucosamine; Hex; hexose; HexNAc; N-acetylhexosamine; HPAEC-PAD; high-performance-an
The role of glycophosphatidylinositol anchor in the amplification of the scrapie isoform of prion protein in vitro
Keywords: Transmissible spongiform encephalopathy; Prion; Prion protein; Conformational conversion; Glycophosphatidylinositol anchor; GPI; glycophosphatidylinositol; NBH; normal hamster brain homogenate; PI-PLC; phosphatidylinositol-specific phospholipase C; PK; pr
Down modulation of IFN-γ signaling in alveolar macrophages isolated from smokers
Keywords: AM; alveolar macrophage; LPS; bacterial lipopolysaccharide; IFN-γ; interferon-γ; IFN-γR; IFN-γ receptor; IRF-1; interferon regulatory factor-1; NSCLC; non-small cell lung cancer; PNGase F; peptide N-glycosidase F; SCLC; small cell lung cancer; SOCS; s
Carbohydrate moieties on the in vitro immunoreactivity of soy β-conglycinin
Keywords: Soy (Glycine max); Immunoreactivity; β-Conglycinin; Deglycosylation; PNGase F
α-cleavage of the prion protein occurs in a late compartment of the secretory pathway and is independent of lipid rafts
Keywords: ADAM; a disintegrin and metalloprotease; Endo H; Endoglycosidase H; ER; endoplasmic reticulum; GPI; glycosyl-phosphatidylinositol; PBS; phosphate-buffered saline; PNGase F; peptide N-glycosidase F; PrPC; cellular form of prion protein; PrPSc; infectious,
Multidimensional system enabling deglycosylation of proteins using a capillary reactor with peptide-N-glycosidase F immobilized on a porous polymer monolith and hydrophilic interaction liquid chromatography–mass spectrometry of glycans
Keywords: Enzyme reactor; Immobilization; Monolith; Immunoglobulin; PNGase F; Glycomics
The Kv1.2 potassium channel: The position of an N-glycan on the extracellular linkers affects its protein expression and function
Keywords: Kv; voltage-gated potassium channel; K+; potassium ion; ER; endoplasmic reticulum; CHO; Chinese hamster ovary cells; Lec1; the glycosylation-deficient CHO mutant cells; Endo H; endoglycosidase H; PNGase F; peptide N-glycosidase F; NXT; N-glycosylation con
Cyclic AMP-dependent protein kinase A and protein kinase C phosphorylate α4β2 nicotinic receptor subunits at distinct stages of receptor formation and maturation
Keywords: protein kinase A; protein kinase C; neuronal nicotinic receptor; phosphorylation; receptor formation; receptor function; ACh; acetylcholine; DMEM; Dulbecco's modified Eagle's medium; ECL; enhanced chemiluminescence; Endo-H; endoglycosidase-H; PAGE; polyac
Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis
Keywords: CA-074; N-(l-3-trans-propylcarbamoyloxirane-2-carbonyl)-l-isoleucyl-l-proline; CREG; Cellular repressor of E1A-stimulated genes; E-64; N-(l-3-trans-carboxyoxirane-2-carbonyl)-l-leucyl-(4-guanidino)-butane; endo H; endo-β-N-acetyl-glucosaminidase H; ER; e
Complex oligosaccharides are N-linked to Kv3 voltage-gated K+ channels in rat brain
Keywords: Kv; voltage-gated K+ channel; Endo H; Endoglycosidase H; CDG; Congenital disorders of glycosylation; PNGase F; peptide N glycosidase F; Glycosylation; Topology; K+ channel; Brain; Congenital disorder of glycosylation;
Engineered yeast with PNGase F on cell surface for releasing of N-glycans from glycoproteins
Keywords: PNGase F; Yeast; Surface display; Biocatalyst