کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7607841 | 1493366 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis
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کلمات کلیدی
DP4N-linked glycansDP3DP6PNGase FDP5RNase BmaltotetraosePeptide-N-glycosidase FRibonuclease BCapillary electrophoresis - الکتروفورزمویرگیOligosaccharides - الیگوساکارید هاisomaltotriose - ایزومالوتوریوزLabeling - برچسب زدنMass spectrometry - طیف سنجی جرمیmaltohexaose - مالتهکسازMaltoheptaose - مالتوئپاتوزMaltopentaose - مالتپنتوزhexahistidine - هگزاشیستیدین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis](/preview/png/7607841.png)
چکیده انگلیسی
The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS). Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigate the effect of the size of labels and a number of charges on CE-MS analysis. Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation, therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydrazone formation chemistry. This labeling approach significantly improved sensitivity with LOD of labeled maltopentaose determined to be 40 nmol/L and also significantly reduced separation time of neutral maltooligosaccharides and N-linked glycans released from bovine ribonuclease B. Furthermore, the labeling by this multi-cationic peptide hydrazine tag also allowed performing analysis of acidic glycans by CE-MS in a positive ion mode as demonstrated by separation of sialylated N-linked glycans released from bovine fetuin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1560, 27 July 2018, Pages 91-96
Journal: Journal of Chromatography A - Volume 1560, 27 July 2018, Pages 91-96
نویسندگان
Jan Partyka, Jana Krenkova, Richard Cmelik, Frantisek Foret,