کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8324412 | 1539909 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Glycogenes mediate the invasive properties and chemosensitivity of human hepatocarcinoma cells
ترجمه فارسی عنوان
گلیکوژن ها باعث می شوند که خواص تهاجمی و خواص شیمایی حساسیت سلول های هپاتوکرارینوما انسان را به خطر بیندازند
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کلمات کلیدی
PBSTBSPBSTPNGase FTumor inhibition rateDMSO - DMSON-Glycan - N-گلیکانPBS containing 0.1% Tween 20 - PBS حاوی 0.1٪ Tween 20Tris buffered saline - تریس نمک بافرInvasion - تهاجمChemosensitivity - حساسیت شیمیاییDimethyl sulfoxide - دیمتیل سولفواکسیدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریGlycogene - گلیکوژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Aberrant cell-surface glycosylation patterns are present on tumors and have been linked to tumor progression. This study aimed to identify the alterations of glycogene and N-glycan involved in tumor invasion, tumorigenicity and drug resistance in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines, which have high, low metastatic potential, respectively. Using real-time PCR for quantification of glycogene and FITC-lectin binding for glycan profiling, we found that the expression of glycogenes and glycan profiling were different in MHCC97-H cells, as compared to those in MHCC97-L cells. We silenced the expression levels of glycogenes MGAT3 and MGAT5, which were over-expressed in MHCC97-L and MHCC97-H cells. Knockdown of MGAT3 expression promoted MHCC97-L cells invasion and increased resistance to 5-fluorouracil in vitro. The silencing of MGAT5 in MHCC97-H cells inhibited invasion and increased sensitivity to 5-fluorouracil in vitro. Further analysis of the N-glycan regulation by tunicamycin application or PNGase F treatment in MHCC97-H and MHCC97-L cells showed partial inhibition of N-glycan glycosylation, decreased invasion, tumorigenicity and increased sensitivity to 5-fluorouracil both in vitro and in vivo. These findings suggest that alterations of glycogene and N-glycan in human hepatocarcinoma cells correlate with tumor invasion, tumorigenicity and sensitivity to chemotherapeutic drug, and have significant implications for the development of treatment strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 2, February 2013, Pages 347-358
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 2, February 2013, Pages 347-358
نویسندگان
Rui Guo, Lei Cheng, Yongfu Zhao, Jianing Zhang, Chunqing Liu, Huimin Zhou, Li Jia,