کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872193 1074110 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of glycophosphatidylinositol anchor in the amplification of the scrapie isoform of prion protein in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The role of glycophosphatidylinositol anchor in the amplification of the scrapie isoform of prion protein in vitro
چکیده انگلیسی
Transmissible spongiform encephalopathies are associated with an autocatalytic conversion of normal prion protein, PrPC, to a protease-resistant form, PrPres. This autocatalytic reaction can be reproduced in vitro using a procedure called protein misfolding cyclic amplification (PMCA). Here we show that, unlike brain-derived PrPC, bacterially-expressed recombinant prion protein (rPrP) is a poor substrate for PrPres amplification in a standard PMCA reaction. The differences between PrPC and rPrP appear to be due to the lack of the glycophosphatidylinositol anchor in the recombinant protein. These findings shed a new light on prion protein conversion process and have important implications for the efforts to generate synthetic prions for structural and biophysical studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 22, 19 November 2009, Pages 3671-3675
نویسندگان
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