کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1948757 | 1054709 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans Purification and characterization of a galactose-specific lectin with mitogenic activity from pinto beans](/preview/png/1948757.png)
A galactose-specific dimeric lectin from pinto beans was purified using a procedure that involved affinity chromatography on Affi-gel blue gel, anion exchange chromatography on Q-Sepharose, fast protein liquid chromatography (FPLC)-ion exchange chromatography on Mono S, and FPLC-gel filtration on Superdex 200. The molecular mass of this homodimeric lectin was 62 kDa and that of each of its subunits was 31 kDa. The hemagglutinating activity of pinto bean lectin was stable within the pH range of 3–12 and the temperature range of 0–70 °C. By using the [3H-methyl]-thymidine incorporation assay, it was shown that the lectin had the ability to evoke a mitogenic response from murine splenocytes but it did not inhibit proliferation of L1210 leukemia cells. The pinto bean lectin inhibited HIV-1 reverse transcriptase with an IC50 of 3 μM.
Journal: Biochimica et Biophysica Acta (BBA) - General Subjects - Volume 1760, Issue 5, May 2006, Pages 808–813