کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1949268 1537739 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Involvement of lipid droplets in hepatic responses to lipopolysaccharide treatment in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Involvement of lipid droplets in hepatic responses to lipopolysaccharide treatment in mice
چکیده انگلیسی


• Hepatic lipid droplets (hLD) from mice are metabolically heterogeneous.
• LPS treatment prevents fasting-induced lipid accumulation in hLD.
• hLD-cholesterol reduction in endotoxemia is related to its export in lipoproteins.

Infection and inflammation induce important changes in lipid metabolism, which result in increased free fatty acids and triacylglycerol in plasma and altered high density lipoprotein (HDL) metabolism. Our aim was to elucidate whether hepatic lipid droplets (LDs) are involved in the adaptations of lipid metabolism to endotoxemia. We characterized the lipid content and several enzymatic activities in subcellular fractions and subpopulations of LDs from livers of mice 24 h after lipopolysaccharide (LPS) treatment and analyzed the expression of key genes involved in lipid management. Endotoxemic mice showed lower lipid content in LDs with decreased molar fraction of cholesteryl ester and higher diacylglycerol/triacylglycerol ratio as compared to their controls. They also showed a decrease in cytosolic triacylglycerol hydrolase activity, specifically in dense LDs, and in microsomal and cytosolic diacylglycerol hydrolase activity; concomitantly neutral lipid biosynthetic capacity and triacylglycerol levels in plasma lipoproteins increased. Together with the overexpression of genes involved in lipogenesis and HDL formation our results suggest that altered hepatic management of LD lipids in LPS-treated mice might be related to the channeled mobilization of triacylglycerol for very low density lipoprotein assembly and to the induction of cholesterol export.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1831, Issue 8, August 2013, Pages 1357–1367
نویسندگان
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