Diacylglycerol stimulates acrosomal exocytosis by feeding into a PKC- and PLD1-dependent positive loop that continuously supplies phosphatidylinositol 4,5-bisphosphate

► Diacylglycerol (DAG)-induced acrosomal exocytosis requires PKC and PLD1 activation. ► DAG promotes a continuous production of phosphatidylinositol 4,5-bisphosphate (PIP2). ► PIP2 is required for IP3 production and intraacrosomal calcium efflux. ► DAG activates Rab3A leading to the assembly of SNARE complexes and membrane fusion.