High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-acylethanolamines

► We present a high-throughput methodology for eicosanoids and N-acylethanolamines. ► The rationale behind the method design is addressed. ► Quantitation for 100 eicosanoid and 33 N-acylethanolamine species. ► Limits of detections are determined. ► Biological application of the methodology is demonstrated.