کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
195000 459804 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a ligation-based impedimetric DNA sensor for single-nucleotide polymorphism associated with metabolic syndrome
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Development of a ligation-based impedimetric DNA sensor for single-nucleotide polymorphism associated with metabolic syndrome
چکیده انگلیسی

Single-nucleotide polymorphism (SNP) detection is becoming important in molecular diagnostics, clinical assay, and novel drug development. Electrochemical methods are well suited for the DNA diagnostics system. Since electrochemical reactions directly emit an electronic signal, expensive signal transduction equipment is not required. We describe the development of a novel DNA sensor that utilizes impedance spectroscopy and DNA ligation reaction on a gold electrode. Impedance spectroscopy enables label-free detection and is nondestructive and useful in equivalent circuit models for interpretation on an electrode surface, whereas from the ligation reaction, the specificity is derived by the allele-specific oligonucleotide of the capture probe on immobilized gold electrode. In other words, DNA diagnostics system using the combination of impedance spectroscopy and ligation reaction is simple, rapid, and allele specific. In this report, we have described a ligation-based impedimetric DNA sensor and the analysis of Trp64Arg polymorphism in the beta3-adrenergic receptor gene (ADRβ3).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 51, Issue 28, 15 September 2006, Pages 6367–6372
نویسندگان
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