کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1950290 | 1537820 | 2006 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Analysis of the 5-lipoxygenase promoter and characterization of a vitamin D receptor binding site
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کلمات کلیدی
Retinoid X receptorMM65-hydroperoxyeicosatetraenoic acid5-HPETE5-LORXRRLUVDREVDRTGFβHDAC1,25(OH)2D3 - 1،25 (OH) 2D31,25-dihydroxyvitamin D3 - 1،25-دی هیدروکسوییتامین D35-Lipoxygenase - 5-لیپوکسیژنازElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزchromatin immunoprecipitation - ایمن سازی کروماتینTransforming Growth Factor Beta - تبدیل بتا فاکتور رشدTransforming growth factor β - تبدیل فاکتور رشد βEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکvitamin D responsive element - عنصر پاسخگو ویتامین DMono Mac 6 - مونو مک 6relative luminescence units - واحد لومینسانس نسبیVitamin D - ویتامین دیPromoter - پروموترCHiP - چیپVitamin D receptor - گیرنده ویتامین D
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and transforming growth factor beta (TGFβ) potently induce 5-lipoxygenase (5-LO) in myeloid cells. We analyzed vitamin D receptor (VDR) binding to putative vitamin D response elements within the 5-LO promoter and analyzed its function by reporter gene analysis. Binding of VDR and retinoid X receptor to the promoter region was shown in DNase I footprinting, electrophoretic mobility shift and chromatin immunoprecipitation assays. However, the identified VDR binding region did not mediate induction of reporter gene activity by 1,25(OH)2D3/TGFβ, neither in the 5-LO promoter context nor with the thymidine kinase (tk) promoter. Insertion of the rat atrial natriuretic factor VDRE in reporter plasmids containing the 5-LO promoter diminished induction by 1,25(OH)2D3/TGFβ as compared with the tk promoter. Similarly, low inductions were obtained when cells were transiently or stably transfected with constructs containing various 5-LO promoter regions. Concerning basal promoter activity, we identified a positive regulatory region (â779 to â229), which includes the VDR binding region, in 5-LO-positive MonoMac6 cells. In summary, the VDR/RXR complex binds to putative VDREs in the 5-LO promoter, but other sequences outside the 5-LO promoter seem to be responsible or additionally required for the prominent induction of 5-LO mRNA expression by 1,25(OH)2D3 and TGFβ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 7, July 2006, Pages 686-697
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 7, July 2006, Pages 686-697
نویسندگان
Bernd L. Sorg, Niko Klan, Sabine Seuter, David Dishart, Olof RÃ¥dmark, Andreas Habenicht, Carsten Carlberg, Oliver Werz, Dieter Steinhilber,