کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1950300 1537820 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lung phospholipid metabolism in transgenic mice overexpressing peroxiredoxin 6
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Lung phospholipid metabolism in transgenic mice overexpressing peroxiredoxin 6
چکیده انگلیسی

Previous studies with peroxiredoxin 6 (Prdx6) null mice demonstrated that the phospholipase A2 activity of this enzyme plays a major role in lung phospholipid metabolism. This study evaluated lung phospholipid metabolism in transgenic mice that over-express Prdx6. Lung lysosomal type PLA2 activity in transgenic mice was 222% of wild type in lung homogenate and 280% in isolated lamellar bodies. Total phospholipid, phosphatidylcholine (PC) and disaturated PC were decreased approximately 20–35% in bronchoalveolar lung fluid, lung homogenate, and lung lamellar bodies in transgenic mice although lung compliance and type 2 cell ultrastructure were unaltered. To study metabolism, unilamellar liposomes (3H-DPPC: PC: cholesterol: PG, 10: 5: 3: 2 mol fraction) were instilled endotracheally in anesthetized mice and lungs were removed for perfusion. Compared to wild type, transgenic mice showed similar net uptake of liposomes in 2 h, but significantly increased 3H-DPPC degradation (38.9 ± 1.1 vs. 29.0 ± 1.3% of recovered dpm). The PLA2 competitive inhibitor MJ33 decreased degradation to 15% of recovered dpm in both transgenic and wild type lungs. Incorporation of [14C] palmitate into DSPC at 24 h after its intravenous injection was markedly increased in both the lung surfactant (+100%) and lamellar bodies (+188%) while incorporation of [3H] choline was increased by only 10–20%. These results indicate increased DPPC degradation and synthesis by the reacylation pathway with Prdx6 overexpression and provide additional evidence that the PLA2 activity of Prdx6 has an important role in lung surfactant turnover.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1761, Issue 7, July 2006, Pages 785–792
نویسندگان
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